Just when tensions looked like they were cooling down, Cardona made a squinting gesture to a few South Korean players.
The incident took place early in the second half during an altercation between a number of players after Kim Jin-su and James Rodriguez clashed.
Colombian footballer Edwin Cardona has apologized for his offensive gesture during his team's friendly match with South Korea on Friday, which was caught on camera and made public, prompting accusations of racism.
South Korea would go on to win 2-1 with Cristian Zapata scoring Colombia's lone goal.
Cardona, who is on now loan at Boca Juniors from Monterrey, was filmed pulling his eyes towards opposition players during the match.
"I didn't see that gesture and I can not comment on that", Pekerman told reporters after the match. "I can't comment further on an incident that I didn't see with my own eyes".
Cardona has since apologised for his actions, in a video posted on the Colombia national team's Twitter account.
"The Colombians were really physical, which can happen in football", said Ki, who plays for Swansea City in the Premier League.
"But racist behaviour is absolutely unacceptable".
"I want to say I am not an aggressive person, I am sorry that something that happened during the game has been misinterpreted".
"Colombia have world-class players, and it was disappointing for the team to show such actions", Ki added.
Colombia heads to China for another World Cup warm-up match on Tuesday, while South Korea hosts Serbia.