They host AS Roma in the first leg of their Champions League quarter-final on Wednesday, one of seven games in the space of 22 days.
Here's a look at some key stats and facts ahead of the game. "I would like it if he didn't have any problems, but it's normal at this stage of the season".
The players called to give blood and urine samples were Marc-Andre ter Stegen, Gerard Pique, Ivan Rakitic, Sergio Busquets, Andres Iniesta, Suarez, Messi, Ousmane Dembele, Jordi Alba, Sergi Roberto and Samuel Umtiti.
Luis Suarez missed Barca's 2-0 win over Athletic Club prior to the worldwide break, but the Uruguay striker is back from suspension this weekend.
Inevitably, he was asked about Leo Messi's fitness but rejected the idea that the club forced the player to sit out Argentina's recent friendlies: "He has been experiencing a few niggles, he could have suffered a tear and it's just not worth the risk".
Lionel Messi returned to training with Barcelona on Thursday to offer the Catalans a boost with less than a week to go before their Champions League clash against Roma. Every player likes to win.
With Busquets still sidelined, Paulinho and Ivan Rakitic are likely to feature in central midfield.
Sevilla, meanwhile, has tumbled out of the Champions League places and sits in sixth place following a pair of consecutive losses prior to the worldwide break.
Sevilla boss Vincenzo Montella, who will be without suspended midfielder Pablo Sarabia, has no intention of adapting his side's style of play for Barca's visit.
In the United States, the game can be watched live on television on beIN SPORTS and kicks off at 14:45 ET. We are looking after him in excatly the same way he was looked after with his national team. They had not lost at all in their previous 21 (eight under Eduardo Berizzo, 13 with Jorge Sampaoli). "He has a little discomfort and we have to bear that in mind, because if it leads to a muscular tear then the situation is far more serious".
The Blaugrana have gone 36 consecutive games without losing in La Liga, only two away from Real Sociedad's record in 1979-80.
"Argentina, with Messi, are more powerful". However, only eight of those have come at the Sanchez Pizjuan.