Caster Semenya's time of 1:56.68 saw her comfortably surpass the previous mark, which had stood for 16 years, although she was still some way short of breaking the oldest track world record in athletics - set in 1983.
Semenya won her first major 1500m title in style on Tuesday when she broke Zola Budd's 34-year-old record with a time of 4:00.71.
But the dual Olympic champion is confident. "To just go out and show the world what I can do".
"We talk about the world record, probably it will be maybe three months or four months from now, but I can't guarantee that", she said.
New Zealanders flocked to the Carrara Stadium hoping for two gold medals, with Valerie Adams targeting a fourth consecutive Commonwealth Games title in the shot put and Eliza McCartney among the favorites in the women's pole vault.
His Games record run of 8:10.07 was only Kenya's second gold of the Games to go with Wycliffe Kinyamal's win in the 800m. "If I had left them behind they might not have pushed".
South Africa's Semenya, who already had the 1500m gold in her suitcase, finished nearly two seconds clear of Kenyan Margaret Nyairera Wambui and Jamaican Natoya Goule. "She's young, she's strong, she's in shape".
The night's last event, the women's 100m hurdles, was expected to be Australia's moment.
With Pearson watching in the stadium, Oluwatobiloba Amusan of Nigeria won in a meet record 12.68 from Jamaicans Danielle Williams and Yanique Thompson.
After competing in seven disciplines in two days, England's Katarina Johnson-Thompson won the heptathlon with 6255 points, a margin of 122 over silver-medalist Nina Schultz of Canada.
As he approached the final water jump, he was waving Abraham Kibiwott on and then stepped out into lane two to allow his teammate the inside running, while Amos Kirui was in a battle with Canada's Matthew Hughes for bronze. Hughes was fourth at Glasgow four years ago as well. "That's why I was telling them in the last 200 metres".